Bacterial Media
From ChoanoWiki
Bacto Marine Broth
This is for growing up marine bacteria. I've approximated this by dissolving just the peptone, yeast extract and Iron citrate in 1L Artificial Sea Water, and it seems to work very well. Note, the Iron Citrate takes a long time to dissolve (hours whilst stirring) (Mark)
Bacto Marine Broth (DIFCO 2216)
| Bacto peptone | 5 | g |
| Bacto yeast extract | 1 | g |
| Fe(III) citrate | 0.1 | g |
| NaCl | 19.45 | g |
| MgCl2 (dried) | 5.9 | g |
| Na2SO4 | 3.24 | g |
| CaCl2 | 1.8 | g |
| KCl | 0.55 | g |
| Na2CO3 | 0.16 | g |
| KBr | 0.08 | g |
| SrCl2 | 34 | mg |
| H3BO3 | 22 | mg |
| Na-silicate | 4 | mg |
| NaF | 2.4 | mg |
| (NH4)NO3 | 1.6 | mg |
| Na2HPO4 | 8 | mg |
| Distilled water | 1000 | ml |
